| Date | Domicile | Score | Extérieur | ||
| 1er Août 2012 – 01h00 | New York Red Bulls (E-U) | 1 – 2 | Tottenham Hotspur (ANG) | ||
| 04h30 | San Jose Earthquakes (E-U) | 2 – 2 | Swansea City (GAL) | ||
| 13h00 | Wrexham F.C. (GAL) | 1 – 3 | AEK Athènes (GRE) | ||
| 16h00 | Atalanta Bergame (ITA) | 0 – 1 | AC Lumezzane (ITA) | ||
| 17h00 | AZ Alkmaar (P-B) | 3 – 1 | Cambuur Leeuwarden (P-B) | ||
| – | PFC Litex Lovech (BUL) | Annulée | Botev Plovdiv (BUL) | ||
| 17h30 | Slavia Sofia (BUL) | 2 – 0 | Apollon Limassol (CHY) | ||
| – | Lokomotiv Sofia (BUL) | 0 – 1 | Atromitos FC (GRE) | ||
| 18h00 | Udinese (ITA) | 1 – 2 | Antalyaspor (TUR) | ||
| – | FC Slivnishki Geroi (BUL) | 0 – 1 | Levski Sofia (BUL) | ||
| 18h30 | 1. FC Kaiserslautern (ALL) | 2 – 3 | Bayern Munich (ALL) | ||
| – | Saint-Etienne (FRA) | 0 – 1 | Nice (FRA) | ||
| 19h00 | Royal Anvers FC (BEL) | 3 – 2 | Valenciennes (FRA) | ||
| – | Preußen Münster (ALL) | 2 – 4 | Borussia Dortmund (ALL) | ||
| – | 1. FSV Mayence 05 (ALL) | 2 – 1 | Szombathelyi Haladas (HON) | ||
| – | Bohemians 1905 (RTC) | 2 – 1 | Parme FC (ITA) | ||
| – | ADO La Haye (P-B) | 2 – 1 | FC Volendam (P-B) | ||
| – | Juventus Turin (ITA) | 1 – 1 | Benfica Lisbonne (POR) | ||
| – | FC Utrecht (P-B) | 1 – 1 | Sparta Rotterdam (P-B) | ||
| 19h30 | Sporting Gijón (ESP) | 1 – 1 | Real Valladolid (ESP) | ||
| 20h00 | Gil Vicente (POR) | 6 – 7 | Sporting Braga (POR) | ||
| – | Olimpia Ljubljana (SLO) | 1 – 1 | Galatasaray (TUR) | ||
| 20h30 | Crewe Alexandra (ANG) | 1 – 0 | Huddersfield Town (ANG) | ||
| 20h45 | Burton Albion FC (ANG) | 1 – 3 | Leicester City (ANG) | ||
| – | Crawley Town F.C. (ANG) | 0 – 1 | Charlton Athletic (ANG) | ||
| – | Morecambe (ANG) | 0 – 2 | Bolton Wanderers (ANG) | ||
| – | Peterborough United FC (ANG) | 0 – 2 | Aston Villa (ANG) | ||
| 21h00 | SSC Naples (ITA) | 2 – 0 | Bordeaux (FRA) | ||
| 21h30 | Córdoba CF (ESP) | 1 – 0 | FC Séville (ESP) |